3,6-Dihydroxyflavone suppresses the epithelial-mesenchymal transition in breast cancer cells by inhibiting the Notch signaling pathway.

3,6-Dihydroxyflavone（3,6-DHF）能有效抑制体外和体内乳腺癌（BC）细胞的上皮间质转化（EMT）。研究表明，3,6-DHF有效抑制乳腺癌干细胞（BCSCs）的形成和增殖，从而降低了小鼠NOD/SCID中肿瘤细胞的肿瘤形成能力。体内光学成像显示，3,6-DHF治疗抑制了乳腺癌细胞在体内的肺转移。进一步研究指出，3,6-DHF通过下调Notch1、NICD、Hes-1和c-Myc，减少NICD-CSL-MAML功能性转录单元的形成，从而导致乳腺癌细胞中Notch信号通路的失活。Notch1的过度表达或miR-34a的抑制显著降低了3,6-DHF对EMT、CSCs以及乳腺癌细胞迁移和侵袭的抑制作用。

Cells were cultured in six-well plates until they reached 100% confluence. A vertical or horizontal wound was gently created in monolayers using a 20 μl sterile pipette tip. The cells were then washed for 3 times with growth medium to remove the detached cells, and the medium was added with fresh medium treated with 3,6-DHF. Images were captured using an inverted microscope and camera at designed times to assess the inhibition of wound closure[1].