购物车

全部删除
您的购物车当前为空

Anti-IL17F Monoclonal Antibody-Biotin

产品编号 TMAZ-0015B

Anti-IL17F Monoclonal Antibody-Biotin is a Biotin-conjugated humanized rabbit antibody targeting IL17F. Anti-IL17F Monoclonal Antibody-Biotin can be used in FACS.

产品编号 TMAZ-0015B

Anti-IL17F Monoclonal Antibody-Biotin is a Biotin-conjugated humanized rabbit antibody targeting IL17F. Anti-IL17F Monoclonal Antibody-Biotin can be used in FACS.

规格	价格	库存	数量
20 μg	询价	7日内发货
100 μg	询价	7日内发货

大包装 & 定制

加入购物车

实验操作小课堂

联系我们获取更多批次信息

资源下载

产品操作手册

TargetMol 的所有产品仅用作科学研究或药证申报，不能被用于人体，我们不向个人提供产品和服务。请您遵守承诺用途，不得违反法律法规规定用于任何其他用途。

产品介绍

生物活性

产品描述	Anti-IL17F Monoclonal Antibody-Biotin is a Biotin-conjugated humanized rabbit antibody targeting IL17F. Anti-IL17F Monoclonal Antibody-Biotin can be used in FACS.
Ig Type	Rabbit monoclonal IgG
交叉反应	Human
验证活性	Flow cytometry analysis of IL17-F overexpressed 394F cells with TMAZ-0015B, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line).
应用	ELISA, FACS
推荐剂量	0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA
抗体种类	Monoclonal
宿主来源	Rabbit
亚细胞定位	Secreted
构建方式	This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of IL17-F protein.
纯化方式	Protein A Affinity Purified
性状	Liquid
缓冲液	0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin.
纯度	Greater than 95%, as determined by SDS-PAGE.
研究背景	Effector cytokine of innate and adaptive immune system involved in antimicrobial host defense and maintenance of tissue integrity (PubMed: 21350122). IL17A-IL17F signals via IL17RA-IL17RC heterodimeric receptor complex, triggering homotypic interaction of IL17RA and IL17RC chains with TRAF3IP2 adapter through SEFIR domains. This leads to downstream TRAF6-mediated activation of NF-kappa-B and MAPkinase pathways ultimately resulting in transcriptional activation of cytokines, chemokines, antimicrobial peptides and matrix metalloproteinases, with potential strong immune inflammation (PubMed: 11574464, PubMed: 11591732, PubMed: 11591768, PubMed: 17911633, PubMed: 18684971, PubMed: 21350122, PubMed: 28827714). IL17A-IL17F is primarily involved in host defense against extracellular bacteria and fungi by inducing neutrophilic inflammation (By similarity). As signature effector cytokine of T-helper 17 cells (Th17), primarily induces neutrophil activation and recruitment at infection and inflammatory sites (By similarity). Stimulates the production of antimicrobial beta-defensins DEFB1, DEFB103A, and DEFB104A by mucosal epithelial cells, limiting the entry of microbes through the epithelial barriers (By similarity). IL17F homodimer can signal via IL17RC homodimeric receptor complex, triggering downstream activation of TRAF6 and NF-kappa-B signaling pathway (PubMed: 32187518). Via IL17RC induces transcriptional activation of IL33, a potent cytokine that stimulates group 2 innate lymphoid cells and adaptive T-helper 2 cells involved in pulmonary allergic response to fungi. Likely via IL17RC, promotes sympathetic innervation of peripheral organs by coordinating the communication between gamma-delta T cells and parenchymal cells. Stimulates sympathetic innervation of thermogenic adipose tissue by driving TGFB1 expression (By similarity). Regulates the composition of intestinal microbiota and immune tolerance by inducing antimicrobial proteins that specifically control the growth of commensal Firmicutes and Bacteroidetes (By similarity)

偶联与修饰

偶联	Biotin
其他可选修饰符	APC/PE/AF488/AF55/AF647
可提供抗体种类	4

抗原信息

免疫原	IL17-F
抗原种属	Human
基因ID	112744
Uniprot ID	Q96PD4
研究领域	Immunology Research

化学信息

分子量

150 kDa

存储&运输

储存方式	For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze.
运输方式	For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice.

计算器

摩尔浓度计算器
稀释计算器
配液计算器
分子量计算器

体内实验配液计算器

请在以下方框中输入您的动物实验信息后点击计算，可以得到母液配置方法和体内配方的制备方法：

比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， TargetMol | Animal experiments

一共给药动物 10 只 ，您使用的配方为 5% TargetMol | reagent

DMSO+ 30%PEG300+ 5%Tween 80 + 60% ddH2O. 那么您的工作液浓度为 2 mg/mL 。

母液配置方法: 2 mg 药物溶于 50 μLDMSO TargetMol | reagent

（母液浓度为 40 mg/mL ）, 如您需要配置的浓度超过该产品的溶解度，请先与我们联系。

体内配方的制备方法: 取 50μLDMSO TargetMol | reagent

母液，添加 300 μLPEG300 TargetMol | reagent

混匀澄清，再加 50μLTween 80, 混匀澄清，再加 600μLddH2O TargetMol | reagent

混匀澄清

以上为“体内实验配液计算器”的使用方法举例，并不是具体某个化合物的推荐配制方式，请根据您的实验动物和给药方式选择适当的溶解方案。

剂量转换

对于不同动物的给药剂量换算，您也可以参考更多

Related Tags: buy Anti-IL17F Monoclonal Antibody-Biotin | purchase Anti-IL17F Monoclonal Antibody-Biotin | Anti-IL17F Monoclonal Antibody-Biotin cost | order Anti-IL17F Monoclonal Antibody-Biotin | Anti-IL17F Monoclonal Antibody-Biotin molecular weight