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Anti-VCAM1 Monoclonal Antibody-AF647
产品编号 TMAZ-0012A6
别名 Vascular cell adhesion protein 1, INCAM-100
Anti-VCAM1 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
Anti-VCAM1 Monoclonal Antibody-AF647
Anti-VCAM1 Monoclonal Antibody-AF647
产品编号 TMAZ-0012A6 别名 Vascular cell adhesion protein 1, INCAM-100
Anti-VCAM1 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF647 can be used in ELISA, FACS.
| 规格 | 价格 | 库存 | 数量 |
|---|---|---|---|
| 20 μg | 询价 | 7日内发货 | |
| 100 μg | 询价 | 7日内发货 |
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| 产品描述 | Anti-VCAM1 Monoclonal Antibody-AF647 is a Biotin-conjugated rabbit antibody targeting VCAM1. Anti-VCAM1 Monoclonal Antibody-AF647 can be used in ELISA, FACS. |
| 别名 | Vascular cell adhesion protein 1, INCAM-100 |
| Ig Type | Rabbit monoclonal IgG |
| 交叉反应 | Human |
| 验证活性 | Flow cytometry analysis of VCAM1 overexpressed 400F cells with TMAZ-0012A6, followed by goat anti-rabbit IgG-ABflo 647 (red line). The isotype control is rabbit IgG (black line). |
| 应用 | ELISA, FACS |
| 推荐剂量 | 0.1-0.2 μg/10E6 cells for FACS; 1 ng/μl for ELISA |
| 抗体种类 | Monoclonal |
| 宿主来源 | Rabbit |
| 亚细胞定位 | Single-pass type I membrane |
| 构建方式 | This antibody was produced from a rabbit immunized with purified, recombinant human extracellular domain of VCAM1 protein. |
| 纯化方式 | Protein A Affinity Purified |
| 性状 | Liquid |
| 缓冲液 | 0.22 μm filtered solution in 20 mM HEPES7.4, 150 mM NaCl, 50% glycerol and 0.03% proclin. |
| 纯度 | > 95% as determined by SDS-PAGE. |
| 研究背景 | Cell adhesion glycoprotein predominantly expressed on the surface of endothelial cells that plays an important role in immune surveillance and inflammation (PubMed: 31310649). Acts as a major regulator of leukocyte adhesion to the endothelium through interaction with different types of integrins (PubMed: 10209034). During inflammatory responses, binds ligands on the surface of activated endothelial cells to initiate the activation of calcium channels and the plasma membrane-associated small GTPase RAC1 leading to leukocyte transendothelial migration (PubMed: 22970700). Serves also as a quality-control checkpoint for entry into bone marrow by providing a 'don't-eat-me' stamping in the context of major histocompatibility complex (MHC) class-I presentation (PubMed: 35210567) |
| 偶联 | AF647 |
| 其他可选修饰符 | Biotin/APC/PE/AF488/AF555 |
| 可提供抗体种类 | 4 |
| 免疫原 | VCAM1 |
| 抗原种属 | Human |
| 基因ID | 7412 |
| Uniprot ID | |
| 研究领域 | Immunology Research |
| 分子量 | 150 kDa |
存储&运输
| 储存方式 | For unconjugated and biotin antibody, store at -20°C or -80°C for long term. Avoid repeated freeze-thaw cycles. For APC/PE/AF647/AF555/AF488 conjugated antibody, store at 4°C, do not freeze. |
| 运输方式 | For unconjugated and biotin antibody, shipping with dry ice. For APC/PE/AF647/AF555/AF488 conjugated antibody, shipping with blue ice. |
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比如您的给药剂量是 10 mg/kg ，每只动物体重 20 g ，给药体积 100 μL ， 
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