CHIKV-IN-3 is a potent inhibitor effective against two low-passage CHIKV strains, demonstrating EC50 values of 1.55 μM for CHIKV-122508 and 0.14 μM for CHIKV-6708, respectively. It operates by targeting host cells to disrupt viral replication, while maintaining a minimal cytotoxic liability (CC50 > 100 μM). Additionally, CHIKV-IN-3 has shown a prophylactic effect [1].

CHIKV-IN-3 (compound (E)-42; HeLa CCL2 cells; 0.1-100 μM; 24 hours) inhibits the Chikungunya virus (CHIKV) replication with an EC 50 of 1.55 μM and demonstrates low cytotoxicity ( CC 50 =129.6 μM) [1]. CHIKV-IN-3 (HeLa CCL2 cells; 20, 40, 80 μM; 24 hours) shows the highest degree of inhibition with approximately 3.9-log reduction in viral titre (ca. 99.99% inhibition) at 80 μM [1]. Cell Cytotoxicity Assay [1] Cell Line: HeLa CCL2 cells, HeLa CCL2 cells infected with CHIKV-122508 Concentration: 0.1-100 μM Incubation Time: 24 hours Result: Inhibited the CHIKV replication with an EC 50 of 1.55 μM and demonstrated low cytotoxicity( CC 50 =129.6 μM)