Epothilone A

产品编号 T6490Cas号 152044-53-6

别名 埃博霉素 A, epothile, Epo A

Epothilone A (Epo A) 是紫杉醇和管蛋白聚合物结合的一种竞争性抑制剂，其Ki 值为 0.6-1.4 μM。

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Epothilone A

产品编号 T6490别名 埃博霉素 A, epothile, Epo ACas号 152044-53-6

Epothilone A (Epo A) 是紫杉醇和管蛋白聚合物结合的一种竞争性抑制剂，其Ki 值为 0.6-1.4 μM。

规格	价格	库存
1 mg	¥ 223	现货
5 mg	¥ 448	现货
10 mg	¥ 708	现货
25 mg	¥ 1,430	现货
50 mg	¥ 2,190	现货
100 mg	¥ 3,290	现货
200 mg	¥ 4,480	现货
1 mL x 10 mM (in DMSO)	¥ 488	现货

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产品介绍

生物活性

产品描述	Epothilone A (Epo A) is a microtubule-stabilizing agent with EC0.01 of 2 μM.
靶点活性	Tubulin:2 μM(EC0.01)
体内活性	Epothilone A诱导微管蛋白聚合, 导致细胞周期在G2-M期停滞, 具有细胞毒性,也诱导细胞凋亡。Epothilone A有效抑制HCT116细胞增殖,IC50为4.4 nM。Epothilone A 对KB3-1, KBV-1, Hela和 Hs578T细胞具有细胞毒性,IC50为13 nM到160 nM。Epothilone A与紫杉醇竞争性与微管结合,IC50为2.3 μM。
激酶实验	Tubulin polymerization assay: Samples for electron microscopy are placed on carbon-over-Parlodion-coated grids (300 mesh) and negatively stained with 2% uranyl acetate. Microtubule assembly in the presence or absence of Epothilone A is monitored spectrophotometrically by using a spectrophotometer equipped with a thermostatically regulated liquid circulator. The temperature is held at 35 °C and changes in turbidity (representative of polymer mass) are monitored at 350 nm. Effective concentration (EC0.01), defined as the interpolated concentration capable of inducing an initial slope of 0.01 OD/min rate, is calculated using the formula EC0.01 = concentration/slope.
细胞实验	Cell lines: KB3-1,KBV-1,Hela,and Hs578T cells. Concentrations: 0–2 μM. Method: cells are plated either in 48-well plates (for trypan blue and cell counting) or onto coverslips.After 24 hours,cells are treated with Epothilone A and are scored at regular intervals.For the cytotoxicity analysis,cells are counted and scored as trypan blue positive or negative.
别名	埃博霉素 A, epothile, Epo A

化学信息

分子量	493.66
分子式	C₂₆H₃₉NO₆S
CAS No.	152044-53-6

储存&溶解度

存储

Powder: -20°C for 3 years | In solvent: -80°C for 1 year | Shipping with blue ice.

溶解度信息

H2O: < 1 mg/mL (insoluble or slightly soluble)

Ethanol: 92 mg/mL (186.4 mM)

DMSO: 92 mg/mL (186.4 mM)

溶液配制表

Ethanol/DMSO

	1mg	5mg	10mg	50mg
1 mM	2.0257 mL	10.1284 mL	20.2569 mL	101.2843 mL
5 mM	0.4051 mL	2.0257 mL	4.0514 mL	20.2569 mL
10 mM	0.2026 mL	1.0128 mL	2.0257 mL	10.1284 mL
20 mM	0.1013 mL	0.5064 mL	1.0128 mL	5.0642 mL
50 mM	0.0405 mL	0.2026 mL	0.4051 mL	2.0257 mL
100 mM	0.0203 mL	0.1013 mL	0.2026 mL	1.0128 mL