HSP27 inhibitor J2 (J2) (J2) is a HSP27 inhibitor, inhibits a production of HSP27 giant polymers, thereby having an effect of inhibiting a chaperone function of the HSP27 and reducing a cell protection function.

HSP27 inhibitor J2与氨甲蝶呤在辐射诱导的肺炎症反应中的比较研究中显示，J2处理显著抑制了肺组织中的免疫细胞浸润，显示了J2的抗炎潜力。通过微型CT分析评估的J2处理小鼠的正常肺体积。J2处理的小鼠逆转了辐射诱导的呼吸困难。然而，氨甲蝶呤与J2相比未显示显著的辐射防护效果。在HSP27转基因小鼠中，我们观察到与野生型小鼠相比，免疫细胞招募增加且正常肺体积减少。辐射后增加的ROS产生和氧化应激经J2处理后得到下调，证明了J2的抗氧化特性。J2可能是治疗辐射诱导肺损伤的有效疗法[1]。

Male C57BL/6 mice (age, 6 weeks; weight, 20–25 g), and were acclimatized (n = 5 per cage) for a week before irradiation. A single dose of 75 Gy was delivered to the left lung in a single fraction using image-guided small-animal irradiator (X-RAD 320) that was equipped with a collimator system composed of 3.5-cm-thick copper to produce focal radiation beams, as well as an imaging subsystem consisting of a fluorescent screen coupled to a charge-coupled-device camera. We selected 3-mm collimators to mimic clinical SBRT conditions by irradiating only a small volume of tissue. The mice were divided into six groups (n = 6–8 per group) as follows: (1) control (C);(2) irradiation (IR) - mice were exposed to a single dose of 75 Gy delivered to the left lung in a single fraction; (3) irradiation + J2 (IR + J2) ?15 mg/kg of J2 was intraperitoneal administered on every other day after irradiation;(4) irradiation + amifostine (IR + Ami) ?100 mg/kg of amifostine were intraperitoneal administered on every other day after irradiation; (5) J2 only (J2) ?15 mg/kg of J2 were intraperitoneal administered on every other day without irradiation; (6) Amifostine only (Ami) ?100 mg/kg of amifostine were intraperitoneal administered on every other day without irradiation. On day 14, mice were sacrificed by CO2 asphyxiation, and their lung tissues were collected for analysis[1].