CD47 Protein, Rat, Recombinant (His)

产品编号 TMPY-03093

CD47 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P97829-1.

CD47 Protein, Rat, Recombinant (His)

产品编号 TMPY-03093

CD47 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P97829-1.

规格	价格	库存
100 μg	¥ 3,820¥ 2,215.6	现货
200 μg	¥ 6,530	5日内发货
500 μg	¥ 13,220	5日内发货

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生物活性

生物活性	Measured by its binding ability in a functional ELISA. Immobilized Rat CD47-His at 10 μg/ml (100 μl/well) can bind mouse SIRPA-Fc , The EC50 of mouse SIRPA-Fc is 0.6-1.4 μg/ml.
产品描述	CD47 Protein, Rat, Recombinant (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 15.2 kDa and the accession number is P97829-1.
种属	Rat
表达系统	HEK293 Cells
标签	C-His
蛋白编号	P97829-1
别名	CD47 molecule
蛋白构建	The Rat CD47 (P97829-1) (Met1-Lys140) was expressed, fused with a polyhistidine tag at the C-terminus.
蛋白纯度	> 90 % as determined by SDS-PAGE
分子量	15.2 kDa (predicted)
内毒素	< 1.0 EU/μg of the protein as determined by the LAL method.
缓冲液	Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS, pH 7.4. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
复溶方法	A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
存储	It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式	In general, Lyophilized powders are shipping with blue ice.
研究背景	CD47 contains 1 Ig-like V-type (immunoglobulin-like) domain and is a receptor for the C-terminal cell binding domain of thrombospondin. It may play a role in membrane transport and signal transduction. CD47 is also a membrane protein, which is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to extracellular matrix. It is very broadly distributed on normal adult tissues, as well as ovarian tumors, being especially abundant in some epithelia and the brain. CD47 may play a role in membrane transport and/or integrin dependent signal transduction. It may prevent premature elimination of red blood cells. It also may be involved in membrane permeability changes induced following virus infection. By acting as an adhesion receptor for THBS1 on platelets, CD47 plays a role in both cell adhesion and in the modulation of integrins. It also plays an important role in memory formation and synaptic plasticity in the hippocampus.Cancer ImmunotherapyCo-inhibitory Immune Checkpoint TargetsImmune CheckpointImmune Checkpoint Detection: AntibodiesImmune Checkpoint Detection: ELISA AntibodiesImmune Checkpoint Detection: WB AntibodiesImmune Checkpoint TargetsImmunotherapyTargeted Therapy

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