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tPA Protein, Mouse, Recombinant (His)

产品编号 TMPK-00810

Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the
predominant route for enhancing cell survival is via an mTORdependent mechanism.

tPA Protein, Mouse, Recombinant (His)

tPA Protein, Mouse, Recombinant (His)

产品编号 TMPK-00810
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
规格价格库存数量
100 μg¥ 2,960¥ 1,716.8现货
500 μg¥ 11,8005日内发货
1 mg¥ 19,7005日内发货
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生物活性

生物活性
Measured by its ability to cleave a peptide substrate, Z-Gly-Gly-Arg-AMC TFA (Cat#T8556). Read at excitation and emission wavelengths of 380 nm and 460 nm (top read). The specific activity is 3861 pmol/min/µg.
产品描述
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.
种属
Mouse
表达系统
HEK293 Cells
标签C-His
蛋白编号P11214
别名
PLAT,Reteplase,t-PA,tPA,Alteplase
蛋白构建
Ser33-Gln559, which consists of two chains: chain A (Ser33-Arg308) and chain B (Ile309-Gln559)
蛋白纯度
> 95% as determined by Tris-Bis PAGE
tPA Protein, Mouse, Recombinant (His)
分子量60.57 kDa (predicted). It migrates to 13-15 kDa (chain A and chain B fragment), 30-34 kDa and 35-40 kDa based on Tris-Bis PAGE result.
内毒素< 1 EU/μg by the LAL method.
缓冲液Lyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
复溶方法
Reconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
存储
It is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.
运输方式In general, Lyophilized powders are shipping with blue ice.
研究背景
Tissue plasminogen activator (tPA) is the predominant plasminogen activator present in the vascular and nervous systems.t tPA is not only neuroprotective for postnatal primary cortical neurons, but also that the predominant route for enhancing cell survival is via an mTORdependent mechanism.

计算器

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  • 重组蛋白稀释 计算器
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