Ganciclovir (2'-Nor-2'-deoxyguanosine) is an ACYCLOVIR analog that is a potent inhibitor of the Herpesvirus family including cytomegalovirus. Ganciclovir is used to treat complications from AIDS-associated cytomegalovirus infections.

FHV-1:5.2 μM

在成年大鼠中，ganciclovir的内耳扩散显示其浓度可达到与血液相同水平。在妊娠期的小鼠中，观察到跨胎盘扩散中胎儿对母体血液的比值为0.5。新生小鼠内ganciclovir的血浆浓度剖面在2小时后出现高峰，随后逐渐下降。在成年小鼠中，该浓度在1小时达到高峰，但在注射后2小时变得无法检测。在接受ganciclovir治疗的新生小鼠中，白细胞、红细胞和血小板的数量显著下降[7]。

For assessment of Akt protein kinase activity in vitro, substrate (2 μg histone H2B or 25 μg eNOS peptide) is incubated with Akt immunoprecipitated from cell lysate using goat polyclonal anti-Akt1 antibody. Kinase reactions are initiated following the addition of reaction components to a final concentration of ATP (50 μM) containing 10 μCi of 32P-γATP, dithiotreitol (1 mM), HEPES buffer (20 mM, pH 7.4), MnCl2 (10 mM), MgCl2 (10 mM). After incubation for 30 min at 30°C, phosphorylated histone H2B is visualized after SDS-PAGE (15%) and autoradiography. To estimate the extent of 32P incorporation into eNOS peptides, each reaction mixture is measured by spotting onto phosphocellulose disc filter and the amount of phosphate incorporated is measured by Cerenkov counting. The wild-type peptide sequence is 1174-RIRTQSFSLQERHLRGAVPWA-1194, and the mutant eNOS peptide is identical except that serine 1179 is substituted by alanine.