Didox (NSC-324360) is a synthetic ribonucleotide reductase (RR) inhibitor that has been found to reduce the levels of oxidative injury markers in the brains of HIV patients with dementia.

Didox引起细胞死亡，而这一效应通过补充铁质得到抑制。Didox处理导致细胞铁含量、TfR1和铁蛋白水平发生变化，这与铁螯合剂deferoxamine（DFO）和deferiprone（DFP）造成的效果相当。Didox是一种双齿铁螯合剂，具有两个理论上可能的结合位点，其中以catechol基团的两个羟基作为配体的是更可能的一种。Didox的铁螯合特性可能通过阻断Tyr122上酪氨酸自由基的形成（如HU）以及通过捕获RRM2（对其活性至关重要）和细胞增殖所需的铁，从而对其抗肿瘤活性贡献。

Didox处理小鼠源骨髓诱发的肥大细胞(BMMC)时，降低了IgE刺激下的脱颗粒反应和包括IL-6、IL-13、TNF及MIP-1a (CCL3)在内的细胞因子产生[2]。

The cells were seeded in a 96-well plate (at a density of 2 × 10^3 cells for HA22T/VGH;?1.5 × 103 cells for HuH7) and exposed to various concentrations of didox and only HA22T/VGH also to hydroxyurea, DFO or DFP (0, 1, 10, 25, 50, 100, 200 and 500 μM) for 24, 48 and 72 h. In other experiments, HA22T/VGH were seeded in 96-well plates and treated with a single dose of didox, HU, DFO, DFP alone or in combination with increasing doses of FAC (25, 50, 100, 200 and 400 μM) for 48–72 h. In other type of treatment,?HA22T/VGH cells were or pre-treated for 16 h with a single dose of didox (200 μM) and then treated in combination with FAC (400–800 μM) or directly in combination didox-FAC for 48–72 h.Cell viability was evaluated with an MTT assay.?After the indicated time points and treatments, the supernatant was removed and 100 μL of the MTT solution (0.5 mg/mL) diluted in the cell medium was added to the wells.?After 3.5 h of incubation at 37 °C and 5% CO2, the MTT medium was removed and 75 μL of DMSO was added to each well.?Plates were shaken for 15 min at 37 °C until complete dissolution and absorbance was measured at 540 nm emission wavelengths.?Average percentage of cell viability at each concentration was calculated using Microsoft Excel 2016 software[1].