流感病毒蛋白 (Influenza Proteins)

Influenza A H1N1 (A Michigan 45 2015) Neuraminidase NA (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.2 kDa.

Influenza A H1N1 (A New York 1 1918) Hemagglutinin Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.5 kDa and the accession number is Q9WFX4.

Influenza A H5N1 (A Cambodia R0405050 2007) Hemagglutinin HA Protein (HA1+HA2, uncleaved, GRRKKR to TETR, aa 1-531, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 60 kDa and the accession number is B6EBW8.

Influenza A H3N2 (A Victoria 361 2011) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is L0HR89.

Influenza A H7N9 (A Anhui 1 2013) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.6 kDa.

Influenza A H1N1 (A Beijing 22808 2009) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is D3YSI0.

Influenza A H1N1 (A WSN 1933) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.1 kDa and the accession number is B4URE7.

Encapsidates the genome in a ratio of 1 N per 6 ribonucleotides, protecting it from nucleases. The nucleocapsid (NC) has a helical structure. The encapsidated genomic RNA is termed the NC and serves as template for transcription and replication. During replication, encapsidation by N is coupled to RNA synthesis and all replicative products are resistant to nucleases. Human parainfluenza 1 virus (HPIV-1) Nucleoprotein NP Protein (His) is expressed in E. coli expression system with N-10xHis tag. The predicted molecular weight is 63.6 kDa and the accession number is P24304.

Encapsidates the negative strand viral RNA, protecting it from nucleases. The encapsidated genomic RNA is termed the ribonucleoprotein (RNP) and serves as template for transcription and replication. The RNP needs to be localized in the host nucleus to start an infectious cycle, but is too large to diffuse through the nuclear pore complex. NP comprises at least 2 nuclear localization signals that are responsible for the active RNP import into the nucleus through cellular importin alpha beta pathway. Later in the infection, nclear export of RNPs are mediated through viral proteins NEP interacting with M1 which binds nucleoproteins. It is possible that nucleoprotein binds directly host exportin-1 XPO1 and plays an active role in RNPs nuclear export. M1 interaction with RNP seems to hide nucleoprotein's nuclear localization signals. Soon after a virion infects a new cell, M1 dissociates from the RNP under acidification of the virion driven by M2 protein. Dissociation of M1 from RNP unmasks nucleoprotein's nuclear localization signals, targeting the RNP to the nucleus.

Influenza A H3N2 (strain A X-31) Polymerase acidic Protein (His) is expressed in E. coli expression system with N-6xHis tag. The predicted molecular weight is 18.6 kDa and the accession number is Q9IQ47.

Binds and inhibits the conjugation of the ubiquitin-like G1P2 ISG15 protein to its target proteins. Since G1P2 ISG15 is an early antiviral protein, NS1 may inhibit the host antiviral response. Prevents EIF2AK2 PKR activation, either by binding double strand RNA or by interacting directly with EIF2AK2 PKR. Also binds poly(A) and U6 snRNA.

Influenza B (strain B Lee 1940) Nucleoprotein NP Protein (His) is expressed in yeast with N-6xHis tag. The predicted molecular weight is 63.8 kDa and the accession number is P04665.

Binds to sialic acid-containing receptors on the cell surface, bringing about the attachment of the virus particle to the cell. This attachment induces virion internalization either through clathrin-dependent endocytosis or through clathrin- and caveolin-independent pathway. Plays a major role in the determination of host range restriction and virulence. Class I viral fusion protein. Responsible for penetration of the virus into the cell cytoplasm by mediating the fusion of the membrane of the endocytosed virus particle with the endosomal membrane. Low pH in endosomes induces an irreversible conformational change in HA2, releasing the fusion hydrophobic peptide. Several trimers are required to form a competent fusion pore.

Forms a proton-selective ion channel that is necessary for the efficient release of the viral genome during virus entry. After attaching to the cell surface, the virion enters the cell by endocytosis. Acidification of the endosome triggers M2 ion channel activity. The influx of protons into virion interior is believed to disrupt interactions between the viral ribonucleoprotein (RNP), matrix protein 1 (M1), and lipid bilayers, thereby freeing the viral genome from interaction with viral proteins and enabling RNA segments to migrate to the host cell nucleus, where influenza virus RNA transcription and replication occur. Also plays a role in viral proteins secretory pathway. Elevates the intravesicular pH of normally acidic compartments, such as trans-Golgi network, preventing newly formed hemagglutinin from premature switching to the fusion-active conformation.

Influenza A H3N2 (A Moscow 10 1999) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.3 kDa and the accession number is Q1NZ37.

Influenza A H6N2 (A chicken Guangdong C273 2011) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.6 kDa and the accession number is I6PXD2.

Influenza A H13N8 (A black-headed gull Netherlands 1 00) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 58.5 kDa and the accession number is Q5DL26.

Influenza A H5N1 (A chicken VietNam NCVD-016 2008) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38.2 kDa and the accession number is C4P278.

Influenza A H11N2 (A thick-billed murre Newfoundland 031 2007) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.4 kDa.

Influenza A H7N7 (A equine Kentucky 1a 1975) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 61.6 kDa and the accession number is B8K0J1.

Influenza A H7N1 (A turkey Italy 4602 99) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 36.5 kDa and the accession number is Q7TAW6.

Influenza A H9N2 (A chicken Hong Kong G9 1997) Neuraminidase NA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.3 kDa and the accession number is Q9Q0U4.

Influenza A H3N2 (A Aichi 2 1968) Matrix protein 1 M1 Protein (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 30 kDa and the accession number is I6SGW5.

Influenza A H3N2 (A Hong Kong 1 1968) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.6 kDa and the accession number is Q91MA7.

Influenza A H5N1 (A common magpie Hong Kong 5052 2007) Hemagglutinin HA1 Protein (HEK293, His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is B7NWR4.

Influenza A H1N1 (A Texas 36 1991) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.1 kDa and the accession number is B4UPL3.

Influenza A H3N2 (A Victoria 208 2009) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa.

Influenza A H2N2 (A Canada 720 2005) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is Q4ZH98.

Influenza A H5N1 (A Indonesia 5 2005) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is A8HWY8.

Influenza A H1N1 (A California 04 2009) Hemagglutinin HA1 Protein(His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is C3W5S1.

Influenza A H1N1 (A USSR 90 1977) Neuraminidase NA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 50.4 kDa and the accession number is P03469.2.

Influenza A H3N2 (A Hong Kong 1 1968) Nucleoprotein NP Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 57.5 kDa and the accession number is P22435.2.

Influenza A H1N1 (A California 07 2009) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59 kDa and the accession number is C3W5X2.

Influenza A H5N1 (A VietNam 1203 2004) Hemagglutinin HA Protein (His & Fc) is expressed in HEK293 mammalian cells with His and Fc tag. The predicted molecular weight is 85.7 kDa and the accession number is Q5EP31.

Influenza A H1N1 (A Brevig Mission 1 1918) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.6 kDa and the accession number is Q9WFX3.

Influenza A H7N7 (A chicken Netherlands 1 03) Hemagglutinin HA Protein (His) is expressed in Baculovirus insect cells with His tag. The predicted molecular weight is 59.4 kDa and the accession number is Q6VMK2.

Influenza A H5N1 (A Anhui 1 2005) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is Q1WDM0.

Influenza A H5N1 (A Anhui 1 2005) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.5 kDa and the accession number is Q1WDM0.

Influenza A H1N1 (A Brisbane 59 2007) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is B0VX44.

Influenza A H1N1 (A California 04 2009) Neuraminidase NA is expressed in HEK293 mammalian cells. The predicted molecular weight is 47.8 kDa and the accession number is C3W5S3.

Influenza A H1N2 (A swine Guangxi 13 2006) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is A5JQC7.

Influenza A H1N1 (A New Caledonia 20 1999) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is Q6WG00.

Influenza A H1N1 (A Solomon Islands 3 2006) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59 kDa and the accession number is A7Y8I1.

Influenza A H5N1 (A goose Guiyang 337 2006) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 59.6 kDa and the accession number is A0FFQ6.

Influenza A H10N3 (A duck Hong Kong 786 1979) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 57.8 kDa and the accession number is A2V811.

Influenza A H16N3 (A black-headed gull Sweden 5 99) Hemagglutinin HA Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 58.6 kDa and the accession number is Q5DL21.

Influenza A H1N1 (A Puerto Rico 8 1934) Hemagglutinin Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 38 kDa and the accession number is P03452.

Influenza A H2N2 (A Canada 720 2005) Hemagglutinin HA1 Protein (His) is expressed in HEK293 mammalian cells with His tag. The predicted molecular weight is 37.7 kDa and the accession number is Q4ZH98.